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KEY SKILLS
 Special Skills Excellent interpersonal, communication skills. Excellent organizational and teaching
skills. Excellent computer skills. Good command of the English language, both written and verbal..
Advanced technical skills in the operation of advance equipments. Interest and facility in biological
research.
 Protein engineering, expression, and purification techniques, including optimization of expression and growth conditions, protein extraction, FPLC, affinity chromatography;
 Molecular biology, with experience in recombinant techniques including PCR, design, construction and purification of recombinant plasmids;
 Cell biology techniques, particularly growing, freezing and maintaining eukaryotic cells in culture;
 Analytical methods including agarose and polyacrylamide gel electrophoresis, DNA sequencing, Western blotting, immunoprecipitation;
 Bioinformatics and use of public DNA/protein sequence and structure databases.
BIOTECHNOLOGY SKILLS
 Mol. Biology/ Biochemical : PCR, RT-PCR, DNA (Mutagenesis, Cloning, Transformation, Extraction & Sequencing), RNA (In-vitro & Reverse Transcription, Isolation, Purification & Characterization), Protein (Expression, Purification & Characterization), Electrophoresis (PAGE, Agarose, SDS-PAGE), Radioisotope Labeling, Footprinting (Enzymatic, Chemical, X-ray dependent, Rapid Quench), ELISA, Western Blot, Northern Hybridization
 Analytical: Spectroscopy (UV/Visible, Fluorescence, NMR, IR), Calorimetry (Thermal Melting, DSC, CD), Chromatography (HPLC, Ion-exchange, Affinity, TLC, GC), Colorimetry, Potentiometry
 Computing: Vector NTI, DNA Strider, NCBI Blast, FASTA, Rasmol, Pymol, Global Melt Fit, M-fold,Origin, Kaleidagraph, Canvas, ChemDraw, Image Quant (IQ), IQ tools, Photoshop, MS Office

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