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Zintro ID: Natale-*****
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Nutraceutical Formulator, Food Scientist and Biochemical Engineer with well-versed background as University Professor and Researcher and Private Biotechnology Consultant with seventeen years of experience in formulating natural products, and developing bio-technologies processes for private Food and Biotech Companies. Consulting experience includes, Equipment Selection and Design for Food and Biochemical processes, Development of Unit Operations, Biochemical analysis and R&D in the areas of Food, Nutraceutical, and Pharmaceutical Sciences. Broad Experience in Formulation, Nanotechnology, Microencapsulation and Isolation of Natural Bioactive Substances. Experience in writing and executing Intellectual Property Patents, trademarks, litigation support services such as patent infringement, forensic investigations and in-depth failure analysis.

 
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Coppell Texas *****

1.- Dr. Eugene Woltering, LSU ; USC Angiogenic Cancer Research Lab, New Orleans LA *****. From ***** to *****.
Isolation and chemical characterization of natural compounds with angiogenic biological activity.

2. Exfuze,LLC
***** U.S Hwy One, Ste 501, Palm Beach Gardens, Florida ***** USA. From ***** to *****.
Director of Research and Development. Development of all quality laboratory control analysis for the manufacture of Nutritional drinks, Bioactive Foods and Nutraceutical products having antioxidant and Immuno-modulatory properties. Development of anti-Inflammatory Nutraceutical Formulations. Worked with Dr. Eugene Woltering ( LSU - AngiogenesisLab) and Dr. Hollie Donze ( LSU-Immunological Lab) testing Bioactive Nutraceutical Compounds.

3.- TRILOGY ESSENTIALS, INC.,
3251 Poplar Ave Suite 15 Memphis, Tennessee ***** USA From ***** to *****
Director of Research and Development.
Developed Nutritional Health formulas for Weight loss, Strengthening the Immune system, Bone and Cartilage Repair and Health Nutraceutical drinks to improve circulatory activity. In addition, developed a Biotechnology process for the encapsulation of amino acids such as Arginine and for the elaboration of Zeolite formulations. Worked in conjunction with Dr. Guoyao Wu of the Department of Animal Science of Texas A & M University in the creation of health products for increasing bio-assimilation of nitric oxide. Also, worked with a parent pharmaceutical company known as Greystone Pharmaceuticals Inc. in creating novel encapsulation systems for specific substance to enhance nitric oxide production.



4.- ALOE COMMODITIES INTERNATIONAL, INC., 1270 Champion Circle Ste. 100, Carrollton, Texas ***** From ***** to *****
Director of Research and Development
Patent author of “Methods for the isolation of Mucilaginous Polysaccharides and uses thereof” Nov 19, 2002. Created several processes for the extraction and biochemical characterization of Peroxides enzyme from natural sources and published as: Peroxides activity from Biopolymer complex isolated from Aloe barbadensis Miller. International Journal of Biotechnology and Biochemistry. Volume 3, Number ***** (2007) pages *****.Characterized and developed a biotechnology process for the production of several important tannin substances; Work published with Dr. Lily Kandra, of the Department of Biochemistry at the University of Debrecen, Hungary on Amylase Inhibition. Work published; Aleppo Tannin: Structural analysis and salivary amylase inhibition. Carbohydr Res., 2007; 342(5): *****. Developed a Nanotechnology procedure for increasing the amount of soluble polysaccharides present in Noni Pulp (Morinda Citrofolia). In cooperation with Dr. Tapan Basu of the Department of Food technology of the University of Alberta Canada, participated in the extraction of steroidal glycosides and glucomanan polysaccharides from Trigonella foenum (fenugreek seeds), and on the isolation of natural occurring polysaccharides from Morinda citrofolia capable of inducing nitric oxide release on human endothelial cell lines.
Director of Research and Development
Formulation of Food and Cosmetic preparations,
Participated in the design of a production facility for the elaboration of health juices and with Dr. Alan Howard formulating products containing Aloe Vera and Creatine (US Patent # 6,168,802: Compositions containing Creatine and Aloe Vera extract, Jan 2, 2001) for the extraction of biologically active substances, from Aloe Vera, and other natural raw materials. Worked with Dr. Anil Ram on the design of a Nutraceutical Company operating under GMP conditions, having fifteen stainless steel compounding tanks, with eight filling lines capable of handling 80,000 bottles per day. Also designed a special flash pasteurization system capable of heating quickly in short time intervals in order to reduce degradation of heat labile substances during hot fill of health juices formulated without preservatives.

5.- ALOE VERA OF AMERICA, INC.***** Jupiter road, Dallas Texas, *****.From *****
Director of Research and Development
Performed R&D on the control of the production of Aloe vera drinks, cosmetic products and other natural formulated health products sold in a parent company named Forever Living Inc, which operated as a multi-level marketing company. Created several fluorometric and NMR analytical techniques for the measurement of active substances such as anthraquinones and Polysaccharides present in natural products.

6.-School of Medicine; Lab of Pathology; UNIVERSITY OF WISCONSIN, Madison, Wisconsin. From *****
Research Assistant
Post-Research Assistant of the School of Veterinary Medicine at the University of Wisconsin. Worked in the isolation of anti-mycobacterial substances isolated from Aloe Vera sap exudates, and which were active against human and animal Tuberculosis. Author of US Patent 5,652,265: Production of Rhein and Rhein Derivates July 29, 1997. Dr. Michael Collins; University of Wisconsin., School of Veterinary Medicine, 2015 Linden Drive, Madison, WI *****.As a Research Assistant of the School Medicine, performed research on the anti-viral activity of Diacetyl Rhein. Reference: Dr. David Pauza; University of Wisconsin, School of Medicine, Department of Pathology 6152 Medical Sciences Center, 1300 University Drive, Madison, WI *****.

7.- REGA INSTITUTE FOR MEDICAL RESEARCH, Leuven, Belgium From *****
Researcher
Worked as researcher, in cooperation with Dr. Erick De Clercq, Director of the Rega Institute for Medical Research in Belgium, on the use of natural occurring substances extracted from medical plants for the inhibition of infectious diseases such as AIDS and human tuberculosis. Work published under the title of: Antiviral Chemistry and Chemotherapy (1994) 5 (5), *****; “Activity of a sulfated polysaccharide extracted from the red seaweed Aghardhiella Tenera against human immunodeficiency virus and other enveloped viruses”. Ref: Dr. Erick De Clercq. Rega Institute for Medical Research, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium.

8.- LABORATORIES ANDROMACO, Madrid, Spain. From *****
Research Scientist
Performed research on the isolation of bioactive substances capable of enhancing biochemical markers such as endocytosis, phagocytosis, nitric oxide production, and TNF-alpha secretion.

9.-Department of Biochemical Engineering. SWISS FEDERAL INSTITUTE, Zurich, Switzerland. From *****
Research Scientist
Design of Air lift Bioreactors for the industrial production of biosurfactans. Cell culture of animal cells for the production of interferon.

10.-Department of Chemical and Biochemical Engineering.UNVERSIDAD METROPOLITANA, Caracas Venezuela. From *****
Associate Professor of Chemical and Biochemical Engineering
Founder of the Biochemical Engineering Research Laboratory at the Department of Chemical Engineering of the Universidad Metropolitana. Biotechnology Consultant of Venezuelan Oil Company PDVSA (Dr. Simon Antunes) and advisor of the Energy & Mines Prime Minister Edwin Arrieta during the period of Government of President Rafael Caldera. Worked in Research and Development on the design of Bioreactors for solid substance fermentation of cellulosic materials and on the creation of emulsions of top crude oils and water using biosurfactants.

University of Wisconsin, Madison, Wisconsin, Post Fellow Research Associate. Departments of Pathology; School of Medicine and Veterinary Medicine, 1994-1996.
Published in cooperation with Dr. Michael Collins the production of antiviral and MMP inhibitor Rhein. US Patent # 5,652,265. Production of Rhein and Rhein Derivates July 29, 1997. Dr. Michael Collins; University of Wisconsin, School of Veterinary Medicine, 2015 Linden Drive, Madison, WI *****.

Universidad Metropolitana.Associate Professor of the Department of Food and Chemical Engineering, Caracas, Venezuela.; Dissertation Research work title: Production of biosurfactants from cultures of Pseudomonas aeruginosa and its use for the formulation of emulsions of top crude oil and water. Sept 16, 1987

Massachusetts Institute of Technology MIT, Cambridge, Massachusetts, 1978 Technology o***** and Industrial Microbiology and Biochemistry.

Postgraduate Master and Bachelor degree in Biochemical Engineering and

Bachelor degree in Biochemistry and Food Science and Technology; Louisiana State University, Baton Rouge, Louisiana State University, Baton Rouge, Louisiana 5/75

American Chemical Society, American Society of Food Technologist, & Ramsey Society of Biochemical Engineers.

List before under Work Experience.
List of Patents
PATENTS
US Patent # 5,652,265. Production of Rhein and Rhein Derivates July 29, 1997. Dr. Michael Collins; University of Wisconsin, School of Veterinary Medicine, 2015 Linden Drive, Madison, WI *****.

US Patent # 6,482,942: “Methods for the Isolation of Mucilaginous Polysaccharides and uses thereof” Nov 19, 2002. Created also processes for the extraction and biochemical characterization of Peroxides enzyme from natural sources and published as: Peroxides activity from Biopolymer complex isolated from Aloe barbadensis Miller. International Journal of Biotechnology and Biochemistry. Volume 3, Number ***** (2007) pages *****.
US Patent # 60,713,421 ; Production of Aloin Isomer A as skin whitening agent from Aloe Vera sap. September 1, 2005.
US Patent # 60,732,030 : Formulation of cosmetics using Garcinia mangostana for the inhibition of metalloproteinases enzymes. Nov. 11, 2005
US Patent # 60,859,126 : Production of Nanoparticles from Fruit juices and Botanical extracts. Dec, 5, 2005.
US Provisional Patent Number: 60,792,031: Method and composition for reducing the formation of heterocyclic amines and acrylamides in fast cooked foods such as hamburgers and French fries. April 14, 2006.
US Provisional Patent Number: 61,127,607: Method for Increasing the Bioavailability of L-Arginine for the production of nitric oxide and uses thereof for the formulation of sport supplements. May 14, 2008.
US Provisional Patent Number : 61,191,752 : Nutraceutical Ice Cream formulations. Oct 11, 2008.
US Provisional Patent Number : 61,284,609 : Method of isolating mucilaginous polysaccharide and uses thereof. 12-22-2009.
US Provisional Patent Number : 61,***** : Method of producing bioactive anti-tumor polyphenols and uses thereof. 03-12-2010.
US Provisional Patent Number : *****,336 : Method of producing Konjac preparation and uses thereof 04-13-2010.
US Provisional Patent Number : ***** : Edulcurant composition having antioxidant activity. 03-13-2011.

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