Expert Profile
• Hands on experience in genomic/plasmid DNA preparation, analysis and cloning/expression in microbes using plasmid, phage and other vectors, site-directed mutagenesis and real-time PCR
• Deft in human and insect cell line culture, transfection and development for recombinant protein production
• Hands on experience in GC, HPLC, NMR, Agarose and polyacrylamide gel electrophoresis, FACS, TEM
• Protein expression, purification, reconstitution, characterization and enzyme kinetics
• ELISA, Immunoprecipitation, Western blot, RNA extraction, cDNA synthesis and microarray analysis
• Maintenance, breeding and genotyping of mice, drosophila and nasonia insects