Molecular Biology
Is it true that "molecular biology has proven that variations in skin color and other presumed racial characteristics develop through recombinations of dna in only a few generations"? I am writing a book and need to cite this statement, but don't remember from where i pulled the quote. Can you help
+2 Other ResponsesCell Biology
This is a full time employment opportunity for a cell biology scientist in the san francisco area.
The successful candidate will play an integral role as a key team member of a group focused supporting our therapeutic candidate discovery efforts as well as on the development and characterization of high productivity manufacturing cho cell lines.
The successful candidate will be highly skilled in cell biology and state-of-art recombinant protein expression technologies. Extensive experience in high titer expression of proteins in mammalian cell culture systems is required. Additional expertise in transfection and selection of stable cell lines, single cell cloning, and evaluation of clonal cell lines for productivity and product quality is highly desired. In addition, knowledge and background in protein biosynthesis and trafficking is a plus.
Responsibilities:
-generate recombinant proteins and antibodies to support discovery research
-troubleshoot/optimize expression of difficult to express recombinant proteins
-perform activities associated with production/manufacturing cell line generation and optimization; including transfection, selection, single cell cloning, high throughput screening, cell banking, etc.
-may support early stage process development efforts
-analyze, interpret and effectively present data at group and team meetings, work collaboratively and cross-functionally to achieve common goals.
-may supervise and mentor scientific staff
required skills:
-highly skilled in cell biology and aseptic cell culture of mammalian cells
-extensive experience in expressing proteins in transient expression and stable systems and knowledge/skills in molecular biology and protein biochemistry are highly desired
qualifications:
-preferred qualification: ph.D. Degree with 1-3 years of relevant postdoctoral experience.
-minimum qualification: master’s degree or bachelor’s degree with 8-10 years of direct relevant experience.
-strong publication record and demonstrated expertise in cell biology, protein expression and related fields.
+57 Other ResponsesMolecular Biology
Need molecular biology expert. For plasmid maxi prep, i started a 100 ml culture in n ampicillin lb broth from a 2 week old 2 ml lb-amp culture from 4 deg c. The 100 ml culture grew well overnight. I did not see a dna pellet after isopropanol step while i saw dna pellets from 3 other simultaneous maxi preps where 100 ml culture was inoculated from fresh 2ml cultures. All plasmids are high copy. I used qiagen endofree maxi prep kit. I need to know why i did not get dna pellet in first plasmid while the 100 ml culture grew well from old 2 ml culture.
+26 Other Responses
