Hi i am doing a project on the sp family which is the sp1 trancription factors. Im working on the mcf7 cell line(breast cancer) and treated with uv radiation at 3 hr, 6 hr and 24hr. And after the uv radiation, the exposed uv radiation cells were given the sp1, p21 and brca1 antibodies to see the effects, and i am using the immunoctochemistry staining for this experiment.
I need help to write up the introduction of the projects in a good strucure logical order with subheadings. Explain about :
sp1 trancription factors(what is it), how can sp1 activity be regulated (1) new synthesis/degradation , increase and decrease total protein (2) altering binding affinity (e;g post translational modification (ptm)
how can sp1 activity be regulated (1) new synthesis/degradation , increase and decrease total protein (2) altering binding affinity (e;g post translational modification)
what is p21?
What is brca1?
How sp1, p21 and brca1 related to each other(link together)
cell stress and dna damage and etc+25 Other Responses
Senior Research Associate Antibody Engineering
Join our team focused on therapeutic antibody discovery and optimization.
-isolate and characterize therapeutic antibodies from both naïve and immune sources
-develop cutting-age technologies for antibody discovery and optimization using phage display and other related technologies
-conduct experiments, record and analyze data
-present results to senior/other research staff
-may supervise other members of the team
-ensure quality standards are met
-stay abreast of latest scientific and technology advancements in the field
-attend team meetings
-ba/bs in biology-related discipline and 10+ years of laboratory experience
-ms in biology-related discipline with 8+ years related laboratory research experience with 5+ years antibody engineering research experience
-experience in a wide range of molecular biology and antibody engineering techniques including pcr, rna isolation, molecular cloning, vector construction, sequencing analysis, mutagenesis, antibody library generation, screening and characterization
-must have experience in protein engineering, directed evolution method such as phage or other display technologies
-requires experience analyzing protein interaction by biacore and related methods
-must have participated as a key member of a multidisciplinary and fast-paced drug hunting team
-exhibit sustained productivity evidenced by high quality publications
-excellent team work and collaboration skills
-demonstrated ability to work in a cross-functional environment
-outstanding written and verbal communication skills
-independent, innovative and creative thinker
-detail oriented with strong organizational and time management skills
-must be able to work in a fast paced, changing environment with demonstrated ability to manage multiple competing responsibilities with a high degree of self-motivation.+8 Other Responses
Currently we are looking for a chinese subject matter expert (sme), specialized in immunology, to check the quality of the translation from the professional point of view (accuracy of the translation and correct and up-to-date terminology are top priorities).
Your task will be to review the english translation of clinical documentation related to antibody technologies and antibody derived therapies and make sure that the translation is accurate and appropriate for the target audience and that the correct terminology is used.
If interested, respond to ****+21 Other Responses