Scientist Molecular Biology/expression
-support antibody cloning, antibody engineering and therapeutic candidate identification
-design and express reagent proteins
-plan, execute and troubleshoot experiments
-examine data and prepare reports
-maintain laboratory including equipment maintenance and ordering of supplies
-master’s degree or bachelor’s degree with 8-10 years of directly related experience
-ph.D. Degree with 0-3 relevant postdoctoral experience preferred
-strong publication record and demonstrated expertise in protein expression and antibody engineering
-highly skilled in molecular biology/dna technology techniques
-extensive experience in v-gene cloning from hybridomas
-experience in restriction enzyme dependent and independent (seamless) cloning
-expertise in antibody engineering, bispecific antibody design and antibody humanization
-knowledge in protein expression in multiple systems and/or purification, a plus
-excellent team work and collaboration skills
-demonstrated ability to work in a cross-functional environment
-outstanding written and verbal communication skills
-independent, innovative and creative thinker
-detail oriented with strong organizational and time management skills
-must be able to work in a fast paced, changing environment with demonstrated ability to manage multiple competing responsibilities with a high degree of self-motivation.+29 Other Responses
Need molecular biology expert. For plasmid maxi prep, i started a 100 ml culture in n ampicillin lb broth from a 2 week old 2 ml lb-amp culture from 4 deg c. The 100 ml culture grew well overnight. I did not see a dna pellet after isopropanol step while i saw dna pellets from 3 other simultaneous maxi preps where 100 ml culture was inoculated from fresh 2ml cultures. All plasmids are high copy. I used qiagen endofree maxi prep kit. I need to know why i did not get dna pellet in first plasmid while the 100 ml culture grew well from old 2 ml culture.+26 Other Responses
Regenerative Medicine Risk Assessment
i am writing on behalf of a consulting firm that conducts expert interviews in support of industry-wide studies for many of the world's fortune 1000 companies. We are an authorized zintro partner.
For a current intriguing study on global stakeholder reactions and risk assessment in regenerative medicine, we are seeking to speak with experts from various groups who could help us understand their perspective on regenerative medicine from an ethical, religious, legislative or social standpoint. We are interested in their opinion around associated challenges in r&d and commercialization of regenerative medicine and future trend and perception of such technology.
We are targeting to speak with experts from one or more of the following groups related to regenerative medicine:
1. Large media groups (e.G. Bbc)
2. Patient groups (e.G. London regenerative medicine network)
3. Payor associations / financial support groups / ngos (e.G. Cancer financial assistance coalition, chronic disease fund, patient access network foundation, etc.)
4. Religious groups & associations (e.G. Muslim jewish forum, religious education group, st. John neumann roman catholic church, etc.)
5. Government organizations (e.G. National health service in the uk, departments of health)
please note that we are only seeking to speak with experts from either the us or the eu5 markets (france, germany, uk, italy and spain), at this stage. We are only interested in personal opinions and are not interested in any confidential information. Our practice is to keep your identity anonymous, as well as that of our client.
We are scheduling 45-60 minute discussions with experts ideally this week (before june 14th), or early next, and are happy to provide a flexible usd 250 compensation for each interview. Please note that we are handling the standard zintro fee separately, hence the expert is not involved in paying this.
If you kindly let me know your contact details (email, phone/skype), i could provide more specifics on the study and standard process.
We would welcome any referrals to other relevant experts as well.
senior specialist, delivery & expert community+17 Other Responses
Start-up master cell bank facility. Seeking an assessment of process plan. They intend to automate as much of the process as possible, but need an expert to assess any gaps in process. Small scale cho cell process, starting from scratch.+6 Other Responses