Dna extraction we extract dna from many many thousands of samples each week, every week for routine snp genotyping. We have found that chilled muscle tissue samples do not perform well when compared to other typical sample types such as buccal swabs, skin biopsies etc. Using inexpensive & easy techniques such as chelex; these fresh muscle tissue samples require a more demanding stringent protocol to achieve equivalent performance. Despite extensive testing we have been unable to develop a simple, robust, inexpensive dna extraction technique for muscle tissue. A first pass pcr success rate of 99% or greater is required. Would love to hear from suitable experts - to suggest potential sources of the problem / solutions? Cost of extraction is a major factor and must be less than $0.20/sample in material costs.
I have over 10 years of experience in this field. Please feel free to reach out for more details. S...2.7 Hours Later
I've done this a few samples at a time using mechanical ***** for high throughput on a...6.7 Hours Later
Laurie |Chair, Department of Health Sciences
Yes, I agree that muscle tissue is not an easy sample for DNA extraction. I have never heard of any ...11.4 Hours Later